prototrophism noun2 •Reversion to the growth factor requirements of an ancestral strain.Berrie [ 254 ] found that the incubation temperature of the colon bacillus has an influence on the incidence of the mutations returning to prototrophism and streptomycin independence — 1962 •The ability to obtain nourishment from a single source, such as iron, sulfur, or sunlight or to undergo anabolism.Bacteria vary greatly in their food requirements from extreme examples of prototrophism and autotrophism to extreme parasitism. — 1934