ayo /ˈeɪ.(j)oʊ/exclamation 3/ˈa.jo/ noun 1 exclamation3 •(US, slang) A greeting."Ayo, Red. It's the homie Spits on the jack for you, blood." "Ayo, homeboy," said Red as he excitedly picked up the telephone receiver. "What's up, fool?" — 2004 •(US, slang) Used to indicate shock towards sth esp. of a disapproving manner. •(US, slang) Used to imply that what was said is inappropriate, perhaps sexually so.Ayo, what'd you just say? ayo /ˈa.jo/ noun1 •A type of mancala strategy game played by the Yoruba.