subculture /ˈsʌbˌkʌltʃɚ/noun 2verb 1forms 3 noun2 •A portion of a culture distinguished by its customs or other features, often in contrast to the larger mainstream culture.The goth subculture has its own mode of dress, and it has a characteristic musical style. •A culture made by transferring microorganisms from a previous culture to a fresh growth medium verb1 •To transfer (microorganisms) to a fresh growth medium in order to start a new cultureCultures were subcultured by 20-fold dilution into fresh MS2D medium approximately every 7 d. — 1990 formssubcultured · subculturing · subcultures