predenaturation

noun1

1. •
   preliminary denaturation

   • Amplification conditions were as following: predenaturation at 94°C for 3 min, 32 cycles of amplification (94°C for 30s, 45°C-72°C for 30s and 72°C for 30s) and a final extension at 72°C for 5 min. — 2015