methylate verb 3noun 1forms 3 verb3 •To add a methyl group to a compound •To add a methyl group to a nucleic acid as part of the process of gene expression •To add, or treat with methyl alcohol (see methylated spirits) noun1 •The anion -O-CH₃⁻ derived from methanol by loss of a proton; any salt containing this anion formsmethylated · methylating · methylates