lady of the night noun4 •(euphemistic) A female prostitute.They call her the lady of the night She's a woman of the world And easy-living girl with love for sale That's what they call her, the lady of the night. — 1976 •Any of certain species of genus Brunfelsia. •Night-blooming cestrum (Cestrum nocturnum), a Central American shrub. •Any member of the species Nyctanthes arbor-tristis. formsladies of the night