5′ adjective 1noun 1forms 2 adjective1 •Relatively close to the 5′ end of a strand of DNA.A CG pair is often designated CpG to signify that the two nucleotides are contiguous in DNA and that the C is 5′ to the G. — 1992 noun1 •That end of any DNA strand that has a terminal phosphate group. formsmore 5′ · most 5′